smooth muscle 22 alpha (sm22-α  (GeneTex)

Journal: BioMed Research International

Article Title: Neonatal Streptococcus pneumoniae Pneumonia Induces an Aberrant Airway Smooth Muscle Phenotype and AHR in Mice Model

doi: 10.1155/2019/1948519

Figure Lengend Snippet: Neonatal S. pneumoniae pneumonia alters airway smooth muscle productions in mice model. IHC staining of lung tissues from both mock-infected (control) and neonatal S. pneumoniae pneumonia mice (S.pp) showing the in situ expression of α -smooth muscle actin ( α -SMA) (a), smooth muscle myosin heavy chain (SMMHC) (f), and smooth muscle 22 alpha (SM22 α ) (k) (400x magnification). The relative α -SMA-positive ( α -SMA + area/Pbm 2 ) (b), SMMHC-positive (SMMHC + area/Pbm 2 ) (g), and SM22 α -positive (SM22 α + area/Pbm 2 ) (l) areas are also shown. RT-qPCR was used to analyze Acta2-mRNA (c), Myh11-mRNA (h), and Tagln-mRNA (m) levels in the lung tissues. The α -SMA (d, e), SMMHC (i, j), and SM22 α (n, o) protein levels in the lung tissues were analyzed by Western blotting. All data are presented as means ± SD. (n =5/group). ∗∗ P<0.01, compared to the control group.

Article Snippet: After blocking endogenous peroxidase activity and non-specific staining with H 2 O 2 and 5% bovine serum albumin (BSA), respectively, the sections were incubated with mouse monoclonal anti- α -SMA, rabbit monoclonal anti-SMMHC, or rabbit monoclonal anti-smooth muscle 22 alpha (SM22 α ) antibodies (all diluted 1:100, Sigma-Aldrich, St. Louis, MO) at 4°C for 12 h. The sections were washed with PBS and incubated with secondary antibody for 30 min at 37°C.

Techniques: Immunohistochemistry, Infection, In Situ, Expressing, Quantitative RT-PCR, Western Blot